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3M™ Emphaze™ AEX Hybrid Purifier

The first fiber chromatographic clarification solution to remove both small insoluble and soluble contaminants.

Reduce cell debris and deliver consistent high purity

Have small soluble contaminants such as DNA and HCP created purification challenges for your downstream process? The solution: 3M™ Emphaze™ AEX Hybrid Purifier is designed to address these challenges and to deliver consistent, high-purity clarified process fluid and to further enhance the performance of Protein A capture column.

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    Excellent throughput and mAb recovery

     

  • bar graph icon with arrow pointing up
    Enhanced capture column performance

     

  • A shield with a check in the middle icon
    Increased capture column protection

     

  • Filtration icon
    High turbidity reduction

     

  • A funnel icon
    DNA, HCP and endotoxin reduction

     


Watch now: One product performing multiple actions

  • Infographic showing 3 benefits of using 3M(TM) Emphaze(TM) AEX Hybrid Purifier

    One step to increase efficiency

    Integrating 3M™ Emphaze™ AEX Hybrid Purifier in the​ clarification stage can improve product purity and​ yield early in the process and may reduce the total​ cost of ownership for the mAb purification process.


A versatile solution to increase process efficiency

3M™ Emphaze™ AEX Hybrid Purifier can be easily incorporated into clarification without changing your current process, in a scalable fashion, and can provide a more efficient process from end to end. It can also replace the second stage of depth filtration.

Product recovery can be substantially increased by reducing the sterilizing membrane surface area pre-Protein A, eliminating the post-viral inactivation depth filter clarification requirement and combining polishing trains into one connected operation.​

​By introducing a fiber chromatographic solutions in your process, you can achieve:

  • Pre-Protein A
    • Nominal 20-40% HCP reduction ​
  • Protein A
    • 10x less residual contaminants
  • Post-Protein A
    • 10x lower HCP
    • 1,000x DNA reduction

Removal of problematic host cell proteins (HCPs) in downsteam mAb purification

  • Cover of  Identification and Tracking of Problematic Host Cell Proteins when using 3M™ Emphaze™ AEX Hybrid Purifier in mAb Manufacturing Processes

    Identification and Tracking of Problematic Host Cell Proteins when using 3M™ Emphaze™ AEX Hybrid Purifier in mAb Manufacturing Processes

    Implementation of 3M™ Emphaze™ AEX Hybrid Purifier during mAb clarification process can substantially enhance purification performance of the Protein A column by significantly reducing DNA and HCP load. In addition, clarification with Emphaze AEX hybrid purifier in conjunction with Protein A chromatography may reduce HCPs that are known to persist through the polishing train. Integration of Emphaze AEX hybrid purifier in the clarification stage may simplify mAb purification by reducing multiple types of impurities in a single process step, leading to better process economics.

    Read the study now (PDF, 1.10 MB)


Endotoxin removal by 3M™ Emphaze™ AEX Hybrid Purifier

  • Cover of Endotoxin removal by 3M™ Emphaze™ AEX Hybrid Purifier

    Read application data for endotoxin removal from protein solutions using 3M™ Emphaze™ AEX Hybrid Purifier - A robust and simple solution to the challenging problem of endotoxin removal to help accelerate the preclinical development of biopharmaceutical drug candidates.

    3M™ Emphaze™ AEX Hybrid Purifier is ideal for endotoxin removal applications in a laboratory setting, especially for high throughput processing of protein samples for screening during pre-clinical and early-stage clinical testing. The high capacity of 3M™ Emphaze™ AEX Hybrid Purifier allows for high protein loads, and scalable products allow for easy transition to pilot or production scale processes.

    Learn more (PDF, 737 KB)


A male scientist with googles and in lab coat is wheeling the 3M encapsulated system holder loaded with 3M encapsulated filters
3M™ Emphaze™ AEX Hybrid Purifier
VIEW PRODUCT DETAILS

Which technology is right for your process?

  • Technology Platforms

    • Quaternary amine (Q) functionalized polypropylene nonwoven
    • 0.2 μm polyether sulfone (PES) membrane

    Mechanism

    AEX

    Materials

    Synthetic

    Feed Stream

    Direct harvest

    Scalability

    Linear scalability from lab to multi-capsule commercial production

    Applications

    • Direct harvest in a single stage
    • Replacement of primary, secondary and guard filters

    Features

    • High mAb product recovery (Capsules: >95%; Conical Tube and Well Plate >90%)
    • Consistent cell loading capacity
    • Turbidity reduction (<15NTU)
    • DNA reduction (<500 ppb)
    • Cell shear minimization
    • 0.1 & 0.2 μm sterile filter protection
    More product details: 3M™ Harvest RC Chromatographic Clarifier
  • 3M™ Emphaze™ AEX Hybrid Purifier

    Technology Platforms

    • Quaternary amine (Q) functionalized polypropylene nonwoven
    • Highly asymmetric 0.2 μm polyamide membrane

    Mechanism

    AEX

    Materials

    Synthetic

    Feed Stream

    Post depth filter, post centrifuge

    Scalability

    Linear scalability from lab to multi-capsule commercial production

    Applications

    • Replacement of secondary depth filters in harvest and clarification
    • Guard filter to Protein A
    • Bioburden control

    Features

    • Nominal 20-40% HCP and greater than 4 logs DNA reduction
    • Consistent output turbidity (<5NTU)
    • Typically, ≥ 4 LRV endotoxin reduction at low conductivity and neutral pH depending on buffer
    More product details: 3M™ Emphaze™ AEX Hybrid Purifier

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Want to simplify your process?

Innovation in cell culture production has created additional complexities for the clarification process. 3M offers single-stage and single-use solutions to help simplify your process and increase manufacturing efficiency.

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Fill out this form for any technical or process-related question, and an appropriate 3M Biopharmaceutical representative will contact you to answer your question or direct your request.


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