3M™ Harvest RC Chromatographic Clarifier

The next-generation solution in harvest and clarification to increase mAb yields

Rising cell densities and increasing levels of soluble and insoluble contaminants of cell cultures has been an ongoing challenge for the bioprocessing industry. If you are still using centrifuge or multi-stage depth filtration, fiber chromatographic clarification may be the answer to meet current and future needs. ​

3M™ Harvest RC Chromatographic Clarifier is a next-generation solution in harvest and clarification technology. It is designed as an efficient option to increase mAb yields while streamlining the clarification process by replacing the centrifuge and/or depth filtration process steps.

  • expansion icon
    Scalability from bench to commercial production​
  • Upward arrow under a magnifier icon
    Predictable performance
  • Recovery icon
    High product recovery
  • Elimination icon
    Whole cell removal and minimize cell sheer
  • Streamline icon
    Streamlined process and smaller footprint

Reimagine your harvest process with simplified and streamlined production

Infographic showing that a 3-step harvest and clarification step is compressed into a single stage process by using 3M™ Harvest RC Chromatographic Clarifier

Anion exchange (AEX) fiber chromatographic technology enables further compression of the process to allow for cost effective manufacturing solutions.

  • Replaces primary and secondary depth filters and guard membranes
  • Removes cells, cell debris, soluble and insoluble contaminates in one step

Experience 3M innovation from lab to production scale

From lab scale to manufacturing, we can help streamline and accelerate your workflow with the entire 3M™ Harvest RC Chromatographic Clarifier portfolio of innovative solutions that enable you to condense steps and improve process efficiencies.

  • Photo of lab scale chromatographic clarifier products
    Discovery phase solutions

    Start your discovery process with lab scale solutions that enable you to do your job in less time with less complexity.

  • Photo of production scale chromatographic clarifier products
    Production scale solutions

    Advance from lab to production scale with the 3M™ Harvest RC Chromatographic Clarifier portfolio of lab and manufacturing capsules.


  • Photo of technician pouring fluid into the bottle top configuration

    Speed up the discovery process with 3M™ Harvest RC Chromatographic Clarifier, BT500

    The latest addition to the 3M™ Harvest RC Chromatographic Clarifier portfolio enables you to do your job in less time with less complexity. It’s an easy-to-use, bottle-top vacuum clarifier that delivers high product recoveries and DNA reduction (<500ppb*) through a streamlined workflow.

  • Fast-processing time (< 10 min.)*

    *Based on 5-8% PCV culture.

  • Single-step clarification for ideal scalability

     

  • Streamline upstream and downstream processes

     

  • Simpler process procedure compared to traditional lab approaches

     


Discover the benefits of 3M™ Harvest RC Chromatographic Clarifier, BT500 in your lab

  • Image comparing the three different approaches for lab harvesting
    High quality samples in less than 10 minutes*

    Compare the speed of clarification in three different approaches to lab harvesting.
    *Based on 5-8% PCV culture.

    Watch the video

  • Image od the 3M™ Harvest RC Chromatographic Clarifier BT500
    See what single-step clarification looks like

    Watch how simple it can be to get started.

    Watch the video


3M science for the unique needs of the discovery phase

Streamline your workflow in the discovery phase with our lineup of lab scaling products, including 3M™ Harvest RC Chromatographic Clarifier, WP6; 3M™ Harvest RC Chromatographic Clarifier, CT15 and 3M™ Harvest RC Chromatographic Clarifier, BT500.

  • Image of 3M™ Harvest RC Chromatographic Clarifier, WP6
    3M™ Harvest RC Chromatographic Clarifier, WP6
    • Well plate
    • Liquid-handling robotic system compatible
    • High purity
    • Simple operation
  • Image of 3M™ Harvest RC Chromatographic Clarifier, CT15
    3M™ Harvest RC Chromatographic Clarifier, CT15
    • Conical tube
    • High-throughput screening
    • High purity
    • High product recovery
  • Image of 3M™ Harvest RC Chromatographic Clarifier, BT500
    3M™ Harvest RC Chromatographic Clarifier, BT500
    • Bottle-top vacuum clarifier
    • Lab-scale single-use chromatographic clarifier
    • Rapid harvesting solution 
    • Delivers high-quality samples for downstream processing      

  • Image of sell sheet

    Enables you to do your job in less time with less complexity

    See more about 3M™ Harvest RC Chromatographic Clarifiers for the lab.

    Discover how your lab can benefit by integrating both speed and efficiency into your process from the start. Learn more about 3M™ Harvest RC Chromatographic Clarifier lab scale solutions.

    Download sell sheet (PDF, 335 KB)


Robust scalability with predictable performance across scales

Fibrous chromatographic clarification assures scalable performance from discovery to manufacturing which allows for faster speed to clinic and market. Performance is consistent from laboratory capsules (BC4 and BC25), to scale-up capsules (BC340 and BC1020), to production capsules (BC2300 and BC16000).

  • Bar chart showing the normalized throughput by various scales of  3M™ Harvest RC Chromatographic Clarifier
  • Bar chart showing the mAb product recovery in % by various scales of  3M™ Harvest RC Chromatographic Clarifier

Biopharmaceutical industry insights

  • Cover of 3M™ Harvest RC Chromatographic Clarifier Beta Testing Series
    See what the industry says about 3M™ Harvest RC Chromatographic Clarifier

    During a Frost & Sullivan Virtual Think Tank Early Access Series, respondents made it evident that they believe adopting 3M™ Harvest RC Chromatographic Clarifier in commercial manufacturing will eliminate key challenges in the harvest process. The multiple benefits of this single-stage, single-use chromatographic clarification solution has driven its industry adoption.

    Read more of Frost & Sullivan's findings (PDF, 934 KB)

  • Cover of Single-stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein production
    Read our recent co-publication with Ambrx

    The use of the 3M™Harvest RC Chromatographic Clarifier, a single-stage chromatographic clarification product, is identified as an alternative strategy to a three-stage filtration train. Using bioprocess cost modeling, the overall cost of the clarification process was reduced by 17%–30% through process compression, depending on bioreactor scale. The benefits of the single-stage harvest process extended through the downstream process, resulting in a 25% relative increase in cumulative yield with comparative impurity clearance.

    Read more


A collection of various sizes of 3M™ Harvest RC Chromatographic Clarifier
3M™ Harvest RC Chromatographic Clarifier

The next-generation solution of harvest and clarification technology to increase mAb yields while streamlining the clarification process.

VIEW PRODUCT DETAILS

Which technology is right for your process?

  • Technology Platforms

    • Quaternary amine (Q) functionalized polypropylene nonwoven
    • 0.2 μm polyether sulfone (PES) membrane

    Mechanism

    AEX

    Materials

    Synthetic

    Feed Stream

    Direct harvest

    Scalability

    Linear scalability from lab to multi-capsule commercial production

    Applications

    • Direct harvest in a single stage
    • Replacement of primary, secondary and guard filters

    Features

    • High mAb product recovery (Capsules: >95%; Conical Tube and Well Plate >90%)
    • Consistent cell loading capacity
    • Turbidity reduction (<15NTU)
    • DNA reduction (<500 ppb)
    • Cell shear minimization
    • 0.1 & 0.2 μm sterile filter protection
    More product details: 3M™ Harvest RC Chromatographic Clarifier
  • Technology Platforms

    • Quaternary amine (Q) functionalized polypropylene nonwoven
    • Highly asymmetric 0.2 μm polyamide membrane

    Mechanism

    AEX

    Materials

    Synthetic

    Feed Stream

    Post depth filter, post centrifuge

    Scalability

    Linear scalability from lab to multi-capsule commercial production

    Applications

    • Replacement of secondary depth filters in harvest and clarification
    • Guard filter to Protein A
    • Bioburden control

    Features

    • Nominal 20-40% HCP and greater than 4 logs DNA reduction
    • Consistent output turbidity (<5NTU)
    • Typically, ≥ 4 LRV endotoxin reduction at low conductivity and neutral pH depending on buffer
    More product details: 3M™ Emphaze™ AEX Hybrid Purifier

Want to simplify your process?

Innovation in cell culture production has created additional complexities for the clarification process. 3M offers single-stage and single-use solutions to help simplify your process and increase manufacturing efficiency.

Ask an expert

Fill out this form for any technical or process-related question, and an appropriate 3M Biopharmaceutical representative will contact you to answer your question or direct your request.


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