3M™ Attest™ Biological Indicators for Steam, 1262-S

The ISO 11138-1:2017 and ISO 11138-3:2017 compliant 3M™ Attest™ Steam Biological Indicator, 1262-S, is easy to use, activate and interpret. It’s a reliable monitoring device for 250°F/121°C gravity displacement and 270°F/132°C vacuum-assisted steam sterilization cycles that is appropriate for use in pharmaceutical and industrial (Life Science) steam sterilization processes when validated by the end user. 

This self-contained biological indicator (BI) consists of a Geobacillus stearothermophilus spore strip and a sealed glass ampoule with growth media and a bromocresol purple pH indicator system. The spore strip and media are contained in a plastic vial topped with a brown color-coded cap. The label includes a chemical process indicator. The BI is packaged in a foil pouch designed to protect the product from humidity excursions.

After the steam sterilization process, the BI is activated to join the growth media with the processed spore strip. The BI is incubated in the 3M™ Attest™ Biological Indicator Incubator 116 for a visual color change readout at 48 hours. A color change from purple to yellow indicates surviving spores and a positive result. When processed, the chemical process indicator turns from a rose red to brown.